ENZYMES & SUBSTRATE CONCENTRATION

==Substrate Concentration== [image:http://i.imgur.com/iywKfbz.png?3] Increasing substrate concentration increases the frequency with which the enzyme and substrate collide. As a result enzyme-substrate complexes form more quickly and the rate of reaction increases. However, there is a limit as eventually there all the enzyme active sites are already occupied with substrate - the enzyme '''active sites become saturated'''. Any further increase in substrate concentration has no further effect on the reaction rate. ==Enzyme Concentration== [image:http://i.imgur.com/hi6hDB8.png?1] Increasing the amount of enzyme also increases the frequency of with which the enzyme and substrate collide. As a result enzyme-substrate complexes form more quickly and the rate of reaction increases. However, there is a limit as eventually there will be more enzyme molecules that substrate. Some enzymes become redundant as they won't have any substrate to bind. Any further increase in enzyme concentration has no further effect on the reaction rate. Note that if there is excess substrate (the first part of the graph) the line can be approximated to a straight line.